Ultrasensitive and rapid enzyme immunoassay (thin aqueous layer immune complex transfer enzyme immunoassay) for antibody IgG to HIV‐1 p17 antigen
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چکیده
منابع مشابه
Enzyme immunoassay for carcinoembryonic antigen.
In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the pe...
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We report an enzyme immunoassay procedure for methotrexate measurement that takes less than 3 h to perform. beta-D-Galactosidase (EC 3.2.1.23) from Escherichia coli was conjugated to methotrexate by means of the mixed anhydride reaction. Bound and free labeled drug were separated by a preincubated cubic complex of first and second antibody. The enzyme activity of the bound fraction was measured...
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متن کاملSerological evaluation of thin-layer immunoassay-enzyme-linked immunosorbent assay for antibody detection in human trichinellosis.
A new immunoenzymatic test, named the thin-layer immunoassay-enzyme-linked immunosorbent assay (TIA-ELISA), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from Trichinella spiralis muscle larvae. Serum samples from people with positive muscle biopsies or symptoms compatible with the disease (n = 8 or 26, respectively), all reactive i...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 1998
ISSN: 0887-8013,1098-2825
DOI: 10.1002/(sici)1098-2825(1998)12:3<179::aid-jcla9>3.3.co;2-b